KARYOTYPES OF FRUIT BATS (CHIROPTERA, PTEROPODIDAE) AND RATS (RODENTIA, MURIDAE) FROM Mt. BAWAKARAENG, SOUTH SULAWESI, INDONESIA

Posted on by

Husni Mubarok, Dyah Perwitasari-Farajallah and Ibnu Maryanto
1Bogor Agricultural University, Faculty of Mathematics and Natural Sciences, W1L5, Dramaga, Bogor, Indonesia
2Indonesia Institute of Sciences, Zoology Division, Research Center for Biology, J1. Raya Jakarta-Bogor KM 46, Cibinong, Bogor, Indonesia Corresponding author; Dyah Perwistasari-Farajallah, Cell; +62 89638616507,

Email; navy.rock@gmail.com

ABSTRACT. This study aims to analyze karyotypes of Sulawesi’s small mammals with Giemsa banding technique. Five fruit bats species (Boneia bidens, Dobsonia viridis, Styloctenium wallacei, Thoopterus nigrescens, Thoopterus suhaniahae) and eight rat species (Bunomys andrewsi, Bunomys chrysocomus, Bunomys heinrichi, Paruromys sp., Rattus dommermani, Rattus exulans, Rattus hoffmanni, Taeromys celebensis) from Mt. Bawakaraeng, South Sulawesi were analyzed. Karyotypes of three species in this study were described for the first time. There were two species of bats and one species of rats showed the best of karyotypes. B. bidens has 2n = 30, FN = 53, FNa = 50, sub metacentric and telocentric for X and Y chromosomes respectively. T. suhaniahae has 2n = 38, FN = 64, FNa = 60 and metacentric X chromosomes. R. hoffmanni has 2n = 44, FN = 61, FNa = 59 and telosentric X chromosomes.

KEYWORD. Karyotypes, 2n, FN, Metacentric, Telocentric

REFERENCES

  • Abramoff, M.D., Magelhaes, P.J., and Ram, S.J. 2004. Image Processing with ImageJ. Biophotonics International 11:36-42.
  • Andersen, K. 1912. Catalogue of the Chiroptera in the collection of the British Museum. Vol 1. Megachiroptera. 2nd edition: British Museum (Natural History), London.
  • Ando, K., Tagawa, K., and Uchida, T.A. 1980. A karyotype study on four species of the Indonesian fruit-eating bats, belonging to Cynopterus, Eonycteris and Macroglossus (Chiroptera: Pteropidae). Journal of Cytology, Cytosystematics and Cytogenetics 33: 41-53.
  • Bakare, A.A., Ademeso, M.M., Adetunji, O.A., and Alabi, O.A. 2011. Pharmaceutical effluent induced chromosome aberration in rat bone marrow cells. Archives of Applied Science Research 3:345-352.
  • Baker, R.J., and Biickham, J.W. 1980. Karyotypic evolution in bats: Evidence of extensive and conservative chromosomal evolution in closely related taxa. Systematic Zool 29:239-253.
  • Baker, R.J., Haiduk, M.W., Robbins, L.W., Cadena, A., and Koop, B.F. 1982. Chromosomal studies of South American bats and their systematic implications. Special Publication Pymatuning Laboratory of Ecology 6: 303–327.
  • Baker, R.J., and Patton, J.L. 1967. Karyotypes and karyotypic variation of North American vespertilionid bats. Journal of Mammalogy 48: 270–86.
  • Husni Mubarok, Dyah Perwitasari-Farajallah and Ibnu Maryanto Bergmans, W., and Rozendaal, F.G. 1988. Notes on collections of fruit bats from Sulawesi and some off-lying islands (Mammalia, Megachiroptera). Zoologische Verhandelingen 248: 1–74.
  • Corbet, G.B., and Hill, J.E. (1992) The Mammals of The Indo-Malayan Region: A Systematic Review: British Museum (Natural History), London. Dobigny, Xuereb A. 2011. Cytogenetics: karyotyping and tissue sampling for fibroblast cell culture.
  • In: Herbreteau, V., Jittapalapong, S., Rerkamnuaychoke, W., Chaval, Y., Cosson, J.F., and Morand, S. (eds.) Protocols for Field and Laboratory Rodent Studies. Kasetsart University Press, Bangkok, pp. 29-30.
  • Duncan, J.F. 1976. Karyotypes of four rats (Rodentia: Muridae) from Sulawesi (Celebes), Indonesia. Cytologia 41: 481 -486.
  • Eldridge, F.E. 1985. Cytognetic of Livestock: Avi Publishing Company Inc., Westport Connecticut, USA. Esselstyn, J.A., Achmadi, A.A. , and Rowe, 2012. Evolutionary novelty in a rat with no molars : A new genus of shrew-rat. Biol. Lett. Published online (http://rsbl.royalsocietypublishing.org/content/early/2012/08/21/rsbl.2012.0574.full).
  • Haiduk, M.W. 1983. Evolution in The Family Pteripodidae (Chiroptera: Megachiroptera) as Indicated by Chromosomal and Immunoelectrophoretic Analyses. [Dissertation]: Graduate Faculty of Texas Tech University, Texas.
  • Haiduk, M.W., Robbins, L.W., Robbins, R.L., and Schlitter, D.A. 1980. Karyotype studies of seven species of African megachiropterans (Mammalia: Pteropodidae). Annals of Carnegie Museum 49:181 -191.
  • Harada, M., and Kobayashi, T. 1980. Studies on the small mammal fauna of Sabah, East Malaysia II. Karyological analysis of some Sabahan mammals (Primates, Rodentia, Chiroptera). Contributions from the Biological Laboratory, Kyoto University 26: 83– 95.
  • Harada, M., Minezawa, M., Takada, S., Yenbutra, S., Nunpakdee,P., Othani, S. 1982. Karyological analysis of 12 species of bats from Thailand. Caryologia 35:269-278.
  • Hood, C.S., Schlitter, D.A., Georgudaki, J.I., Yenbutra, S., and Baker, R.J. 1988. Chromosome studies of bats (Mammalia: Chiroptera) from Thailand. Ann Carnegie Mus 55: 99-109.
  • Laurie, M.O., and Hill, J.E. 1954. List of land mammals of New Guinea, Celebes and adjacent islands 1758-1952: British Museum (Natural History), London. Levan, A., Fredga, K., and Sandberg, A.A 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
  • LIPI, Bappenas, K.L.H. 2014. Buku kekinian keanekaragaman hayati: LIPI (in press), Bogor.
  • Li, Y., Wu, Y., Harada, M., Lin, L.K., and Motokawa, M. 2008. Karyotypes of three rat species (Mammalia: Rodentia: Muridae) from Hainan Island, China, and the valid specific status of Niviventer lotipes. Zoological Science 25: 686-692.
  • Loganathan, E., Anuja, M.R., and Nirmala, M. 2012. Automated identification to the centromere position and the centromer index (CI) of human chromosomes in Gbanded images. IJATER 2: 2250-3536.
  • Maryanto, I., and Higashi, S, 2011. Comparison of zoogeography among rats. fruit bats and insectivorous bats on Indonesian islands. Treubia 38 : 33-52.
  • Maryanto, I., and Yani, M, 2003. The new species of the Rousettus bat from Lore Lindu National Park Central Sulawesi, Indonesia. Mammal Study 28: 111-120.
  • Maryanto, I., Yani, M., Prijono, S.N., dan Wiantoro, S. 2012. A new species of fruit bat (Megachiroptera: Pteropodidae: Thoopterus) from Sulawesi and adjacent islands, Indonesia. Records of The Western Australian Museum 27: 068–084
  • Musser, G.G. 1981. Result of the Archbold expeditions. No. 105. Notes on systematics of Indo-Malayan murid rodents, and descriptions of new genera and species from Ceylon, Sulawesi, and the Philippines 168: 225-334.
  • Mortelliti, A., Castiglia, R., Amori, G., Maryanto, I., and Musser, G. G. 2012. A new species of Margaretamys (Rodentia: Muridae: Murinae: Rattini) from Pegunungan Mekongga, southeastern Sulawesi, Indonesia. Tropical Zoology 25: 74 – 107.
  • O’Brien, S.J., Menninger, J.C, and Nash, W.G. 2006. Atlas of Mammalian Chromosomes: Jhon Wiley & Sons, New York.
  • Sakamoto, Y., and Zacaro, A.A. 2009. LEVAN an ImajeJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes. Initial-version. An open source Java plugin distributed over the Internet from http://rsbweb.nih.gov/ij/.
  • Wilson, A.C., Bush, G.L., Case, S.M. and King, M.C. 1975. Social structuring of mammalian populations and rate of chromosomal evolution. PNAS 72: 5061–5065.
  • Wilson, D.E. and Reeder, D.M. 2005. Mammal Species of the World a Taxonomic and Geographic Reference, 3rd eds.
  • The Johns Hopkins University Press, Baltimore. Yong, H.S. 1984. Robertsonian translocation, pericentric inversion and heterochromatic block in theevolution of the tailless fruit bat. Experientia 40: 875-876.

Download Full Paper Here (Right-Click and Save As.. )