GROWTH PERFORMANCE AND SELECTED BIOCHEMICAL INDICES ASSESSMENT IN THE SERUM AND LIVER OF CLARIAS GARIEPINUS JUVENILES EXPOSED TO VARYING CONCENTRATIONS OF WATER SOLUBLE FRACTION OF CRUDE OIL

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Olumide Isaac Fakolujo1, Henry Adefisayo Adewole2, Efere Martins Obuotor3, and Victor Folorunsho Olaleye2*
1Institute of Ecology and Environmental Studies, Obafemi Awolowo University, Ile-Ife, Nigeria
2Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria
3Department of Biochemistry and Molecular Biology, Obafemi Awolowo University,Ile-Ife, Nigeria

*Corresponding author: volaleye@oauife.edu.ng; +234-803-404-5185

 

ABSTRACT. The effect of varying concentrations (0, 0.25, 0.05 and 1.0% v/v) of water soluble fraction (WSF) of crude oil on the growth performance and biochemical changes in the serum and liver of 144 Clarias gariepinus Juveniles stocked into 12 glass aquaria at the rate of 12 fish per tank in triplicate for 90 days was assessed. The growth performance indices of the fish juvenile over the 90 days of toxic exposure revealed that the mean weight gained, specific growth rate, and total feed intake of the fish specimen decreases with increase concentration of the WSF of crude oil. However, 10% and 20% mortality were recorded in the fish exposed to 0.5% and 1.0% WSF of crude oil respectively. The activity of ALT and AST in the serum of the fish were significantly higher (p<0.05) with the increased concentration of WSF of crude oil while the ALT and AST activity of the liver of the fish juveniles irrespective of the added concentration of WSF of crude oil showed no significant differences (p>0.05). The study concluded that WSF of crude oil had adverse effects on the growth performance, blood serum and liver activities in C. gariepinus juveniles.


KEYWORDS. Crude oil, Growth performance, Serum, Enzymes, Fish, Pollution

 

REFERENCES

  • Abdel-Moneim AM, Abou Shabana NM, Khadre SEM, Abdel-Kader HH. Physiological and histological effects in catfish (Clarias lazera) exposed to dyestuff and chemical wastewater. International Journal of Zoological Research 2008; 4:189-202
  • Adeola MO. Present and potential Sources of Water Pollution Effects on Fish and Wildlife Resources. In Aina E.O.A. and Adefioye, N.O. (Eds). FEPA Monographs 1996; 6:191-197
  • Adewolu MA, Adeoti AJ. Effect of mixed feeding schedules with varying dietary crude protein levels on the growth and feeding utilization of Clarias gariepinus (Burchell, 1822) fingerlings. Journal of Fisheries and Aquatic Science 2010; 5:304-310
  • Afolabi OA, Adeyemi SA, Imevbore AMA. Studies on toxicity of some toxicity of some Nigeria crude oils to some aquatic organisms. In: Proceedings of 1985 Int. Seminar on the Petroleum Industry and the Nigeria Environment. NNPC, Lagos: 1985; 269- 273
  • Baden SP. Oxygen consumption rate of shrimp exposed to crude oil extract. Marine Pollution Bulletin 1982; 13(7):230-233
  • Couch C, John A. Disease, Parasites, and toxic responses of commercial penaeid shrimps of the Gulf of Mexico and South Atlantic coast of North America. EPA-600/J-78-072.
  • U.S. Fish and Wildlife Service. Fishery Bulletin 1978; 76:1 -44.
  • Das P, Ayyapan S, Das B, Jena J. Nitrite toxicity in Indian Major carps: sublethal effect on selected enzymes in fingerlings of Catla catla, Labeo rohita and Cirrhinus mrigala. Comparative Biochemistry and Physiology C-Toxicology and Pharmacology 2004a; 138:3-10
  • Das P, Ayyapan S, Jena J, Das B.. Acute toxicity of ammonia and its sub-lethal effects on selected heamatological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton). Aquaculture Research 2004b; 35:134-143
  • Dede EB, Kaglo HO. Aqua-toxicological effects of water soluble fraction (WSF) of diesel fuel on Oreochromis niloticus fingerlings. Journal of Applied Sciences and Environmental Management 2001; 5(1):93-96
  • Ezenmoye L, Ogbomida TE. Histopathological effects of gammalin 20 on African catfish (Clarias gariepinus) Applied and Environmental Soil Science (2010); 1-8 Ezenwa BI, Ayinla OA. Conservation strategies for endangered fish breeding and nursery grounds within the coastal wetlands of Nigeria. Aquatic Conservation, Marine and Fresh Water Ecosystems 1994; 4:125-133
  • Gauthier L, Tardy E, Mauchet F, Marty J. Biomonitoring of the genotoxic potential (micronucleus assay) and detoxifying activity (EROD induction) in the River Dadau (France), using the amphibian Xenopus laevis. Scientific Total Environment 2004; 323:47-61
  • Heintz RA, Short JW, Rice SD. Sensitivity of fish embryos to weathered crude oil – Part II. Increased mortality of pink salmon (Onchorpynchus gorbuscha) Environmental Toxicology and Chemistry 1999; 18(3):493-503
  • Hodson PV, Maj MK, Efler S, Burnison BK, Van Heiningen ARP, Girard R, Carey JH. Experiment with oil and dispersants on the sea grass Zostera holtii. Report of the Advisory Committee on Pollution of the Sea, Field Study Council London. 1997 Horsefall M, Spiff AI. Principles of Environmental Chemistry, Metropolis Ltd., Port Harcourt .1998; p 112-119.
  • Lin SC, Chung TC, Ueng TH, Lin YH, Hsu SH, Chiang CL, Lin CC. The hepatoprotective effects of Solanum alatum Moench on acetaminophen-induced hepatoxicity in mice. A.M.J. Uin. Med. 2002; 28:105-114
  • Mason CF. Biology of Freswater Pollution, 3rd Ed., Longman and Scientific Technical, Harlow, U.K. 1996; 356 pp
  • Murty AS, Rajabhushanam BR, Ramani AV, Christopher K. Toxicity of fenitrothion to the fish, Mystus cavasius and Labeo rohita. Environmental Pollution Series A: 1983; 30:225-232.
  • Ofojekwu PC, Onah JA. The effects of water soluble fraction of crude oil on the growth of the catfish, Clarias gariepinus (Burchell, 1822). African Journal of Environmental Pollution and Health 2002; 1(2):1-7
  • Palanivelu V, Vijayavel K, Ezhilarasibalasubramanian S, Balasubramanian MP. Influence of insecticidal derivative (Cartap Hydrochloride) from the marine polychaete on certain enzyme systems of the freshwater fish Oreochromis mossambicus. Journal of Environmental Biology 2005; 26:191 -196
  • Powell CB. Effects of freshwater oil spillages on fish and fisheries. In: proceedings of 1987 Seminar on the petroleum Industry and the Nigerian Environment. NNPC, Lagos 1987; 208-227
  • Ramesh M, Sivakumari K, Kanagaraj M. Toxicity of dye effluent in lactate dehydrogenase activity in Labeo rohita. Journal of Environmental Protection 1993; 13:124-127 Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminase. American Journal of Clinical Pathology 1957; 28(1):56-63
  • Schmidt E, Schmidt FW. Determination of serum GOT and GPT. Enzym. Biol. Clin. 1963; 3:1Sunmonnu TO. Biochemical effect of crude oil polluted water on catfish (Clarias gariepinus) and the effect of its consumption on selected rat cellular system. Ph.D. thesis, Department of Biochemistry, University of Ilorin, Ilorin: 2006; 58pp Sunmonu TO, Oloyede OB. Changes in liver enzyme activities in African catfish (Clarias gariepinus) Exposed to Crude Oil Exposure. Asian Fish Science 2006; 19:104-112.
  • Sveier H, Raae AJ, Lied E. Growth and protein turnover in Atlantic salmon (Salmo salar): The effect of dietary protein level and protein particle size. Aquaculture 2000; 185:101-120.
  • Val AL, Almeid-Val VMF. Effects of crude oil on respiratory aspects of some fish species of the Amazon. In: Biology of Tropical Fish (Ed. A.L. Val and V.M.F. Almeida-Val). National Institute for Research in the Amazon, Manaus: 1999; 227-291
  • Olumide Isaac Fakolujo, Henry Adefisayo Adewole, Efere Martins Obuotor, and Victor Folorunsho Olaleye Wake H. “Oil Refineries: A review of their ecological impacts on the aquatic environment”. Estuarine, Coastal and Shelf Science 2005; 62:131-140
  • Wang W. Toxicity test of aquatic pollutants by using common duckweed environmental pollutant. Environ. Poll. 1986; 11:1-14.
  • Wegwu MO, Omeodu SI. Evaluation of selected biochemical indices in Clarias gariepinus exposed to aqueous extract of Nigerian crude oil (Bonny Light). Journal of Applied Science and Environment Management. 2010; 14(1):77-81
  • Weisshaar D, Gossrau E, Faderl B. Normal ranges of alpha-HBDH, LDH, AP and LAP as measured with substrate optimated test charges. Med Welt 1975; 26:387.
  • WWF (World Wildlife Fund) Fishing on the Niger River. 2006; Retrieved May 10, 2007 from
    http://www.panda.org/news_facts/multimedia/video/index.cfm?uNewsID=61121 .
  • Yusuf OA, Adewole HA, Olaleye VF. Assessment of the Water Quality of Saba River, Osogbo, Nigeria. Notulae Scientia Biologicae 2017; 9(2):188-195.

 

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