TOTAL FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITYBY DIFFERENT DRYING AND EXTRACTION METHODS OF Clinacanthus nutans LEAVES

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Mohd Hafiz Abdul Majid, Yvonne Melse Laurence, Mohammad Shaheen Khan  and Mohd Sani Sarjadi 

a Faculty of Science and Natural Resources, Universiti Malaysia Sabah,88400 Kota Kinabalu, Sabah, Malaysia.
b Knowledge and Technology Management Division, Sabah Economic Development & Investment Authority (SEDIA), 88873, Kota Kinabalu, Sabah, Malaysia.

*Corresponding author: msani@ums.edu.my

ABSTRACT. This report presents a study on the total flavonoid content and antioxidant activity of Clinacanthus nutans leaves from different drying and extraction methods. The C. nutans leaves were subjected through three different drying methods: oven (40°C, 60°C and 80°C), vacuum oven and air drying. Afterwards, extraction on the dried leaves was performed using three different extraction techniques: soxhlet, maceration and ultrasoundassisted solvent extraction. Finally, total flavonoid and antioxidantactivity was determined spectrophotometrically by aluminium chloride colorimetric assay and 2-Diphenyl-1-picryl hydrazyl (DPPH) method respectively. The results showed that the choice of drying and extraction methods had a significant influence on total flavonoid and antioxidant content of C. nutans extract. The combination of drying C. nutans leaves using laboratory oven at 60°C and soxhlet extraction obtained the highest amount of total flavonoid and antioxidant content at 24.53 ± 0.95 mg RU/g and 89.73 ± 4.39mg TE/ g respectively. The highest antioxidant activity for C. nutansfromair-dried samples and vacuum oven-dried samples were obtained through maceration extraction at 85.46 mg TE/ g and 83.96 mg TE/ g respectively.

KEYWORDS. Clinacanthus nutans; Drying; Extraction method; Flavonoid; Antioxidant

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