EXTRACTION OF 4H-PYRAN-4-ONE, 2,3- DIHYDRO -6-METHYL-, AN ALTERNATIVE ANTIFUNGAL AGENT, FROM SCHIZOPHYLLUM COMMUNE: OPTIMIZATION AND KINETIC STUDY

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Yi Peng Teoh1,2* and Mashitah Mat Don1

1School of Chemical Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal, Seberang
Perai South, Penang, Malaysia.
2Faculty of Engineering Technology, Universiti Malaysia Perlis (UniMAP), P.O Box 77,
D/A Pejabat Pos Besar, 01000 Kangar, Perlis, Malaysia
Email: teoh.yipeng@gmail.com

ABSTRACT. 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP) was believed as a promising alternative in term antifungal activity towards fungal attack in rubberwood. Solid-liquid extraction is performed from basidiomycetes fungus Schizophyllum commune in methanol-water solvent, in order to obtain valuable antifungal agent. Statistical optimization was employed to optimize the extraction condition for maximal total flavonoid content (TFC) and DDMP productivity. The optimum conditions were 70.75% (v/v) methanol, 29 °C, and 145 rpm. The optimization studies were verified and the experimental data fitted well to the selected models with error percentage less than 1%. The extraction kinetics was then investigated using Parabolic diffusion model, Power law model, Peleg’s model, and Elovich’s model. All empirical models gave a good fit to the experimental data (R2 > 0.9), in which the Power law model having the highest R2 and lowest RMSD values.

KEYWORDS. Schizophyllum commune; total flavonoid content (TFC); 4H-pyran-4-one, 2,3- dihydro-3,5-dihydroxy-6-methyl- (DDMP); optimization; extraction kinetics

 

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